Metabolism of the Fusarium Mycotoxins T-2 Toxin and HT-2 Toxin in Wheat

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Metabolism of the Fusarium Mycotoxins T-2 Toxin and HT-2 Toxin in Wheat

To investigate the metabolic fate of HT-2 toxin (HT2) and T-2 toxin (T2) in wheat (Triticum aestivum L.), an untargeted metabolomics study utilizing stable isotopic labeling and liquid chromatography-high resolution mass spectrometry was performed. In total, 11 HT2 and 12 T2 derived in planta biotransformation products were annotated putatively. In addition to previously reported mono- and digl...

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Metabolism of HT-2 Toxin and T-2 Toxin in Oats

The Fusarium mycotoxins HT-2 toxin (HT2) and T-2 toxin (T2) are frequent contaminants in oats. These toxins, but also their plant metabolites, may contribute to toxicological effects. This work describes the use of 13C-assisted liquid chromatography-high-resolution mass spectrometry for the first comprehensive study on the biotransformation of HT2 and T2 in oats. Using this approach, 16 HT2 and...

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Assay and relationship of HT-2 toxin and T-2 toxin formation in liquid culture.

Both T-2 toxin and HT-2 toxin can be conveniently quantitated in crude extracts by using a combination of thin-layer chromatography and fluorodensitometry. This technique was used to follow the production of these toxins by liquid cultures of Fusarium poae (NRRL 3287). T-2 toxin was produced prior to HT-2 toxin and hexadeuterio-T-2 toxin was converted by the culture to trideuterio-HT-2 toxin.

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In vitro metabolism of T-2 toxin.

Incubation of T-2 toxin with the 9,000 x g supernatant fluid of both human and bovine liver homogenate resulted in conversion to a single, deacetylated product identified as HT-2 toxin. Metabolism is more rapid in human liver. HT-2 toxin was not produced when human plasma was the incubating medium nor was it produced by treatment of T-2 toxin with simulated gastric juice. T-2 toxin was stable i...

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An Investigation on T-2 Toxin Contamination in Wheat Crops from 11 Cities, Iran

Objective: The major Fusarium toxins found in cereals and cereal-based products that can be harmful to both human and animal health are some trichothecenes, such as deoxynivalenol (DON). Methods: A recent SCOOP project, Since toxinogenic fungi are cosmopolitan, mycotoxins are environmental pollutants present in virtually all parts of the world and causing diseases (mycotoxicoses) . Thi...

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ژورنال

عنوان ژورنال: Journal of Agricultural and Food Chemistry

سال: 2015

ISSN: 0021-8561,1520-5118

DOI: 10.1021/acs.jafc.5b02697